The mapping population used in this study (A05141) consisted of 162 F1 progeny derived from a cross between ‘Rio Grande Russet’ (female parent) and ‘Premier Russet’ (male parent). ‘Premier Russet’ is a dual purpose (processing and fresh market) potato variety with significant resistance to the accumulation of reducing sugars after long-term storage at low temperatures . Rio Grande Russet is a high yielding fresh market potato variety . A05141 was developed at the USDA-ARS Small Grains and Potato Germplasm Research Unit (Aberdeen, ID) and was one of the mapping populations phenotyped and genotyped by the Solanaceae Coordinated Agricultural Project (SolCAP) .
Field experiments was indeed conducted from the USDA-ARS, University away from Idaho Lookup and you may Expansion Heart (Aberdeen, ID) and North carolina State School Tidewater Search Channel (Plymouth, NC) during the summer seasons of 2010 and you will 2011. The latest progeny and mothers was examined within the an effective randomized over cut-off design with several replications of 10-mountain plots at each and every location. Vine maturity are visually obtained to your a 1–nine level at 95 (95DAP) and you will 120 (120DAP) weeks shortly after planting, with step 1 = very early, 100% vines inside a story dead, when you find yourself 9 = extremely later, full grow having buds clear. Plant gains behavior was filed at the beginning of blooming and conveyed as step three = erect (> 45° with floor), 5 = semi-upright (30–45° with crushed), and you may seven = spreading (More document 10: Desk S5). Verticillium wilt and you will very early blight (EB) (Altenaria tenuis) diseases was indeed ranked on the an excellent 0–9 measure. To possess EB, 0 suggests no condition and you may 9 > 90% of leaf area affected for the a storyline. Getting VW, the size and style are a blended get to have leaf city/stem flagging as a result of the problem (Extra document ten: Dining table S5). Plots were not inoculated, however, Aberdeen industries have high inoculum of Verticillium from the crushed, accumulated over years. Early blight sometimes appears yearly from the ID and you can NC locations and that it trial the illness are allowed to create definitely, without fungicides applied for their control.
Tuber examples was treated with Isopropyl Chlorpropham (N-(3-chlorophenyl) carbamate) at a rate off twenty two ppm to quit sprouting and you will kept at 42 °F and you will 95% relative humidity to own 90 days.
Sugar density towards treatments within this demo had been calculated regarding a good 10-tuber shot with the style of Sowokinos ainsi que al. with variations. Tubers have been slash using an enthusiastic Kut Footwear Stringer French fry cutter. 2 hundred g from tuber tissue collected in the center out of the new ten tubers was in fact macerated inside the an enthusiastic Acme Juicerator (Acme Equipment, Spring Mountain, FL). While in the control, tuber cells is cleanse with 150 mL out of sodium-phosphate barrier (0.05 M, pH eight.5) to own a last homogenate level of 275 mL. Glucose concentrations have been computed using a YSI design 2700 Analyzer (Red Springs Appliance Co., Inc., Purple Springs, OH) and conveyed to the a percentage new lbs base.
Bud-stop and you may Stalk-stop fry colour
A strip out of cells (step 3.0 cm ? 0.8 cm) taken out of each one of the 10 tubers found in the fresh new glucose extraction procedure was utilized to own fry color devotion (ten pieces for every imitate). Pieces have been fried in canola petroleum at 191 °C getting step 3.5 minute. Fry color is determined within step three minute having fun with a product 577 Photovolt Reflection Meter (design 577, Photovolt Tool Inc., Minneapolis, MN). A green filter out escort backpage Brownsville was used and the software try calibrated using a black-cavity fundamental as the 0.0% reflectance and a white plaque (Pet. Zero. 26-5Cat. Zero. 26–570-08) given that 99.9% reflectance. Proportions have been adopted the fresh new comes to an end each and every remove. The reduced the fresh new reflectance measurement, the new darker the fresh new fry colour and you will vice versa.